TABLE 1.
Genotypes of E. coli and phage T4 strains useda
| Strain | Genotype | Reference |
|---|---|---|
| E. coli | ||
| MCS1 | supD araD139 Δ(ara-leu7697) ΔlacX74 galU galK hsdR rpsL pro (uncharacterized proline auxotrophy) | 19 |
| TabG | E. coli B, nonsuppressing, hsdR gal met tabG | 29 |
| BE-BS | E. coli B, nonsuppressing | 31 |
| Phage T4 | ||
| T4 denAB | denA (nd28) [denB-rII]Δ (rIIPT8) | 2 |
| T4 denAB motAΔ | T4 denAB motAΔ | 2 |
| T4 denAB motA-pc1 | T4 denAB motA-D30A/F31A | This study |
| K10 | T4 denAB 38 (amB262) 51 (amS29) | 31 |
| K10 motAΔ | K10 motAΔ | 2 |
| K10 motA-pc1 | K10 motA-D30A/F31A | This study |
a
The denA and denB mutations inhibit host DNA degradation, and the 38am and 51am mutations block phage growth unless an amber suppressor is present.