TABLE 3.
Burst size analysis of suppressor mutantsa
| T4 strain | Plasmid gene | Burst size |
|---|---|---|
| K10 (motA+ asiA+) | supF | 45 |
| K10 motA-pc1 (asiA+) | supF | 0.4 |
| K10 motA-pc1 asiA-S22F | supF | 2.0 |
| K10 motA-pc1 asiA-Q51E | supF | 1.3 |
| T4 denAB (motA+ asiA+) | rpoD+ | 73 |
| T4 denAB motA-pc1 | rpoD+ | 0.5 |
| T4 denAB motA-pc1 | rpoD-D570N | 2.6 |
| T4 denAB motA-pc1 | rpoD-Y571C | 4.6 |
| T4 denAB motA-pc1 | rpoD-Y571H | 5.5 |
| T4 denAB motA-pc1 | rpoD-L595P | 2.8 |
| T4 denAB motA-pc1 | rpoD-S604P | 2.3 |
a
The host cells for the infections with K10 and its derivatives contained plasmid pBSPLO+, which carries supF and thereby allows suppression of the amber mutations in this phage background. The host cells for the infections with T4 denAB and its derivatives contained a plasmid encoding either wild-type or mutant ς70 (rpoD gene), along with the normal (wild-type) chromosomal rpoD gene.