Figure 4.

Effects of once-daily administration of desHis¹Pro⁴Glu⁹-glucagon or desHis¹Pro⁴Glu⁹-glucagon(Lys¹²PAL) (each at 25 nmol/kg bw) for 18 days on insulin sensitivity (A) in HFF-STZ mice, with the related area above the curve (B) presented. Insulin was administered by i.p. injection at 5 IU/kg/bw in non-fasted mice. Additionally, effects on pancreatic hormone content were assessed on day 18. Pancreatic glucagon (C) and insulin (D) levels were assessed in excised pancreatic tissue via a commercially available ELISA or in-house RIA, respectively. Values are mean ± s.e.m. (n = 8). *P < 0.05, ***P < 0.001 compared to HFF-STZ saline controls. ^ΩP < 0.05, ^ΩΩΩP < 0.001 compared to HFF saline controls.