Figure 5.

Effects of once-daily administration of desHis¹Pro⁴Glu⁹-glucagon or desHis¹Pro⁴Glu⁹-glucagon(Lys¹²PAL) (each at 25 nmol/kg bw) for 18 days on O₂ consumption (A and B), CO₂ production (C and D), respiratory exchange ratio (RER) (E and F) and energy expenditure (G and H) in HFF-STZ mice. Mice were placed in CLAMS metabolic chambers for 18 h to acclimatise, and measurements were obtained over a further 24 h period (12 h dark period as shown by black bar parallel to x-axis) at the end of the treatment period. O₂ consumption and CO₂ production were measured for 30 s at 25 min intervals (A and C). RER was calculated by dividing VCO₂ by VO₂ (E and F). Energy expenditure was calculated using RER with the following equation: (3.815 + 1.232 × RER) × VO₂ (G). Average energy expenditure is also provided (H), separated into the light (LP) and dark phases (DP). Overall incremental data are presented in panels B, D, F and G, where each data point represents information collected at individual time-points over the 24 h period. Values are mean ± s.e.m. (n = 6). **P < 0.01, ***P < 0.001 compared to HFF-STZ saline controls. ^ΩP < 0.05, ^ΩΩP < 0.01, ^ΩΩΩP < 0.001 compared to HFF saline controls.