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. 2022 Sep 21;57:102480. doi: 10.1016/j.redox.2022.102480

Fig. 3.

Fig. 3

METH inhibits CSE expression and eNOS phosphorylation. Skeletal muscle tissue was obtained for mRNA and protein analysis from saline control and METH treated mice. qRT-PCR mRNA measurement was performed for (A) CSE (B) CBS and (C) eNOS. Panel D illustrates representative western blot images for CSE, CBS, phospho-eNOS, and total eNOS. Quantitation of protein expression normalized to tubulin is shown for (E) CSE, (F) CBS, (G) eNOS, and (H) p-eNOS (ser1177). Panel I reports skeletal muscle tissue total sulfide and NOx levels between saline control and METH treated mice. n = 4–7 per cohort, ###P < 0.0001 vs. saline control; ##P < 0.001 vs. saline control; #P < 0.05 vs. saline control.