Table 1.
Susceptibility of HIV Envelope-Expressing CEM Cell Lines to HIV-Mediated Cytopathic Effects
| CD4 Expressiona (mean channel number) | HIV Antigensb (% cells positive) | Cell Viabilityc (%) | Reverse Transcriptase in Culture Supernatants (counts/ml) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cell Line | 0 | 7 | 18 | 0 | 7 | 18 | 0 | 7 | 18 | 0 | 7 | 18 |
| days after infection | ||||||||||||
|
|
||||||||||||
| CEM Infected with pDOL CMV ● env | 240* | 147 | 15 | |||||||||
| 224# | 160 | 25 | 0 | 70 | >95 | >95 | 60 | >97 | 0 | 2–4 × 104 | 3–5 × 105 | |
|
|
||||||||||||
| CEM Control | 540* | 105 | 10 | |||||||||
| 515# | 80 | 20 | 0 | 75 | – | >95 | <35 | <2 | 0 | 1–3 × 105 | 2.5–3 × 106 | |
|
|
||||||||||||
| CEM Infected with pDOL ● tat | 505* | 85 | 30 | |||||||||
| 580# | 100 | 20 | 0 | 60 | – | >95 | <40 | <2 | 0 | 8–10 × 104 | 2–3 × 106 | |
G418 selected cell lines were infected by an over-night incubation in virus containing medium (2 × 105 cells/ml) from CEM producer lines. Titer of virus was standardized as relative dilution that induced HIV antigen positivity in 20%–30% of CEM cells 2 days after infection. Results are representative analysis of multiple experiments (n = 4).
a
Analyzed by fluorescence activated cell sorter analysis using OKT4* and OKT4A# antibodies.
b
Analyzed by indirect immunofluorescence using sera from an HIV seropositive hemophiliac with high gag reactivity (titer 1:2580).
c
Determined by trypan blue exclusion.