Figure 3.

OBP-702 induces immunogenic cell death with HMGB1 release in human PDAC cells

(A and B) Human PDAC cells with WT-p53 (A) and mt-p53 (B) were treated with OBP-301 or OBP-702 at the indicated MOI for 48 h. Supernatants were collected, and the level of extracellular HMGB1 was determined using an HMGB1 ELISA. Uninfected (mock-treated) cells were shown as virus-infected cells at an MOI of 0. Data are expressed as mean ± SD (n = 3). Student’s t test was used to evaluate the significance of differences. ∗p < 0.05; ∗∗p < 0.01 (versus an MOI of 0).