Figure 6.
In vivo anti-tumor effect of combination therapy with OBP-702 and anti–PD-1 antibody in murine PAN02 tumor model mice
(A) PAN02 cells (5 × 106 cells/site) were inoculated into the flanks of C57BL/6J mice. Mice were then injected intratumorally with 1 × 108 PFUs of OBP-702 once weekly for three cycles (green arrowheads). Anti-PD-1 antibody was administered intraperitoneally once weekly for three cycles (blue arrowheads). PBS (black arrowheads) was used as a control. (B) The number of CD8+ and Foxp3+ cells was calculated from five randomly selected fields. Data are expressed as mean ± SD (n = 6). One-way ANOVA followed by the Games-Howell multiple comparison test was used to evaluate the significance of differences. ∗p < 0.05; ∗∗p < 0.01. Representative photographs of immunohistochemical staining for CD8+ and Foxp3+ T cells in each group. Scale bars, 100 μm.