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Two-hybrid assay for in vivo Rep protein interaction. Activation of the lacZ reporter in the two-hybrid yeast strain CTY10/5d transformed with plasmids expressing the LexA and Gal4AD fusion proteins, shown on the left, was measured by a permeabilized cell assay. β-Galactosidase activities represent the averages and standard deviations obtained with four independent transformants. WT, wild type.
