REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
HA Tag Polyclonal Antibody	IGBMC antibody Facility	Rabbit Polyclonal
FLAG Tag Monoclonal Antibody	IGBMC antibody Facility	Mouse Monoclonal
Bacterial and virus strains
Escherichia coli: OP50	Caenorhabditis Genetics Center	OP50
Escherichia coli: HT115(DE3)	Caenorhabditis Genetics Center	HT115(DE3)
Escherichia coli: DH10β	Invitrogen™	DH10β
Escherichia coli: BL21(DE3)	Stratagene	BL21(DE3)
Chemicals, peptides, and recombinant proteins
Proteinase K from Tricachium album	Sigma-Aldrich	Cat#: P2308
Ampicillin	Sigma-Aldrich	N/A
IPTG	GoldBio	Cat#: I2481C
KH2PO4	Sigma-Aldrich	Cat#: P0662
Na2HPO4	Thermo Fisher Scientific	Cat#: S374-3
NaCl	Thermo Fisher Scientific	Cat#: S271-3
MgSO4	Sigma-Aldrich	Cat#: M7506
cOmplete Mini, EDTA-free Protease Inhibitor Cocktail	Millipore Sigma	Cat#: 11836170001
Tetramisole hydrochloride	Sigma-Aldrich	Cat#: L9756-5G
Tricaine	Sigma-Aldrich	Cat#: E10521-10G
NaOH	Sigma-Aldrich
NaCl	Sigma-Aldrich
Tris pH8	Sigma-Aldrich
EDTA	Sigma-Aldrich
Dithiothreitol (DTT)	ThermoFisher Scientific	Cat#: R0861
Critical commercial assays
Taq DNA Polymerase, 5 U/μL	Roche	11 435 094 001
Master Mix Phusion	New England Biolabs	M0531L
Q5® Site-Directed Mutagenesis Kit	New England Biolabs	E0554S
T4 DNA Ligase	New England Biolabs	M0202
mMESSAGE mMACHINE™ T7 Transcription Kit	Invitrogen™	AM1344
NucleoSpin® Gel and PCR Clean-up	Macherey-Nagel	740609.50
NucleoSpin® Plasmid	Macherey-Nagel	740588.50
NucleoBond® Xtra Midi	Macherey-Nagel	740410.50
Hitrap Ni crude 1 mL columns	Dutscher	17-5247-01
NGM agar plates	N/A	N/A
LB agar plates	N/A	N/A
LB	N/A	N/A
Experimental models: Organisms/strains
C. elegans strains: See Table S2
Oligonucleotides
Oligonucleotides: See Table S3	Sigma-Aldrich
Recombinant DNA
CloneJET PCR Cloning Kit	Thermo Fisher Scientific	K1231
pET30a+	Novagen	pET30a+
pET32a+	Novagen	pET32a+
pPD95.75	Fire Kit, Addgene	pPD95.75
pPD97.82	Fire Kit, Addgene	pPD97.82
pPD122.53	Fire Kit, Addgene	pPD122.53
pOD1988	Wang et al., 2017	pOD1988
2nls::gfp	This study	pSJ553
egl-5p(6.5 kb)::nanobodyGFP::zif-1.	This study	pSJ559
lin-17p::2nls::gfp.	This study	pSJ567
lim-6(int4)::gfp	This study	pSJ739
lim-6int4(mutated)::gfp.	This study	pSJ759
let-413a::gfp::pest	This study	pSJ721.14
lin-26p::gfp	This study	pSJ722
T7p::6XHis::hmg-pop-1	This study	pSJ769
T7p::6XHis::sox-2 full::HA	This study	pSJ6094
T7p::6XHis::hmg-pop-1::Flag	This study	pSJ1107
col-34p::gfp::cki-1(gDNA)::unc-54 3′utr	This study	pSJ1108
col-34p::gfp::cki-1(cDNA)::unc-54 3′utr	This study	pSJ1112
egl-5p(1,3 kb)::sox-2 full antisense.	This study	pSJ6293
lim-6(int4)::mCherry	This study	pSJ1096
col-34p::gfp::sox-2(cDNA)::unc-54 3′utr	This study	pSJ6199
Software and algorithms
Adobe Illustrator CS6	http://www.adobe.com/products/illustrator.html	RRID:SCR_010279
Fiji	https://imagej.net/Fiji/Downloads	RRID: SCR_002285
Imaris	https://imaris.oxinst.com/packages	RRID: SCR_007370
GraphPad Prism	https://www.graphpad.com/	RRID: SCR_002798
LAS X	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/	RRID:SCR_013673
SnapGene viewer	http://www.snapgene.com/products/snapgene_viewer/	RRID:SCR_015053
ApE (A plasmid Editor)	https://jorgensen.biology.utah.edu/wayned/ape/	N.A
UCSC Genome Browser	http://genome.ucsc.edu/cgi-bin/hgTracks?db=ce11&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chrX%3A1074694%2D1078639&hgsid=1004849487_ACKae4H3vpcGQxQU9xfEIuAVn6Sj	RRID:SCR_005780
Genomatix Software: MatInspector	https://www.genomatix.de/online_help/help_matinspector/matinspector_help.html	RRID:SCR_008036
ALGEN-PROMO	http://alggen.lsi.upc.es/cgi-bin/promo_v3/promo/promoinit.cgi?dirDB=TF_8.3	RRID:SCR_016926
FIMO	http://meme-suite.org/doc/fimo.html	RRID:SCR_001783
Jaspar	http://jaspar.genereg.net/	RRID:SCR_003030
CIS-BP	http://cisbp.ccbr.utoronto.ca/TFreport.php?searchTF=T333272_2.00	RRID:SCR_017236
Other
Leica MZ6	Leica Microsystems
Leica SP5	Leica Microsystems
Leica DM6 B	Leica Microsystems
HAMAMATSU Digital Camera		C11440-42U30