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. 2022 Sep 12;2022:9827986. doi: 10.34133/2022/9827986

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Copyright © 2022 Feng Chen et al.

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Histological characterization and monosaccharides of the egg envelope in two spawning habits of C. alburnus eggs after fertilization. (a, b) Histological characterization staining with Alcian blue- (pH 2.5) periodic acid Schiff (AB-PAS) in the adhesive and semibuoyant eggs of C. alburnus at 1 h after fertilization (scale bar is 10 μm). Black arrow: outer edge of the 4th layer of the adhesive egg envelope; AE: adhesive egg; BE: semibuoyant egg; EE: egg envelope; CA: cortical alveoli. (c) The monosaccharide analysis of the egg envelopes of the two types of C. alburnus eggs was performed at 0, 0.5, and 1 h after fertilization. Values are presented as the mean ± SEM. ^∗indicates p < 0.05 versus C. alburnus-BE and ^∗∗indicates p < 0.01 versus C. alburnus-BE.