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Co-immunofluorescence analysis demonstrating localization of egg envelope proteins (ZP2 and ZP3X1) in unfertilized eggs (scale bar is 20 μm). (a–e) Adhesive egg of C. alburnus (C. alburnus-AE); (f–j) semibuoyant egg of C. alburnus (C. alburnus-BE); (a, f) stained results with haematoxylin-eosin; (b, g) control groups; (c–e and h–j) immunofluorescence localization of ZP2 (magenta) and ZP3X1 (green) in the egg envelope. EE: egg envelope; CA: cortical alveoli; Yg: yolk globule.
