Skip to main content
. Author manuscript; available in PMC: 2022 Sep 27.
Published in final edited form as: J Vis Exp. 2022 Apr 21;(182):10.3791/63586. doi: 10.3791/63586

Figure 2: The bioanalytical workflow.

Figure 2:

Micro-dissection and capillary aspiration, or FACS facilitated sampling of cellular and clonal protein content. Depletion of abundant yolk proteins and separation by capillary electrophoresis (CE) or nano-flow liquid chromatography (LC) enhanced detection sensitivity using electrospray ionization (ESI) high-resolution mass spectrometry (HRMS). Quantification revealed dysregulation, supplying new information for hypothesis-driven studies. Figures were adapted with permission from references15.