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. 2022 Sep 27;9:54. doi: 10.1186/s40779-022-00412-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — HDAC7-c-Myc positive feedback loop to promote ESCC growth and involves in the anti-proliferative action of melatonin on ESCC cells. a Representative IHC images for c-Myc expression in ESCC tissues. Scale bar, 200 μm and 50 μm (inset), respectively. b Statistical analysis of c-Myc expression in 148 ESCC patients through IHC staining. c Kaplan–Meier survival analysis by high/low c-Myc levels of the 148 ESCC patients based on the microarray tissue IHC results. d Spearman correlation analyses of HDAC7 and c-Myc expression in the ESCC tissues. e Kaplan–Meier analysis of the association between overall survival and the expression of HDAC7 and c-Myc on 148 ESCC patients. f Representative Western blotting results of c-Myc, HDAC7, p21, p27 and Cyclin D1. g Representative Western blotting results of HDACs in the EC109 cells with c-Myc knockdown. h Representative Western blotting results of c-Myc, HDAC7, p21, p27 and Cyclin D1 in the EC109 and EC9706 cells with 10058-F4 treatment. i Representative images and results of colony formation and EdU incorporation assays. j Co-treatment of melatonin, LV-Flag-HDAC7 and LV-shc-Myc #1 was applied to EC109 cells. Representative Western blotting results of HDAC7, Flag-HDAC7, c-Myc, p21 and p27 were shown. ^*P < 0.05 vs. the NC group, ^#P < 0.05 vs. the HDAC7 OE group, ^&P < 0.05 vs. the MEL 4 mmol/L group, ^$P < 0.05 vs. the HDAC7 OE + MEL 4 mmol/L group. HDAC7 histone deacetylase 7, ESCC esophageal squamous cell carcinoma, OE overexpression, LV lentivirus, MEL melatonin, IHC immunohistochemistry