TABLE 1.

Strains and plasmids used in this study

Strain or plasmid	Genotype	Construction or source
Bacillus strains
PY22	trpC2	Laboratory strain
BSA46	trpC2 SPβ ctc::lacZ	3
BSA419	trpC2 SPβ ctc::lacZ PSPAC::rsbT	22
IS1	trpC2 thr-5	27
IS169	trpC2 thr-5 tsp-6 (rplK57)	27
BSZ5	trpC2 SPβ ctc::lacZ relA::Spcr	pUS-RE1→BSA46
BSZ9	trpC2 SPβ ctc::lacZ rplK57	IS169→BSA46
BSZ10	trpC2 SPβ ctc::lacZ PSPAC::rsbT rplK57	BSZ9→BSA419
Plasmids
pUS-19	Apr Spcr	4
pUS-RE1	Apr SpcrrelA	This study