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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Biochemistry. 2021 Nov 11;62(2):410–418. doi: 10.1021/acs.biochem.1c00451

Figure 1.

Figure 1.

Graphical representation of Br512 and the electrostatic force map of HP47. (a) Br512 was constructed by fusing HP47 with a GS linker to the N-terminal of Bst-LF. An 8× His-Tag was added at the N-terminal of the new fusion protein to aid with purification. (b) Models of HP47 electrostatic force using an Adaptive Poisson–Boltzmann Solver to identify surface charge. The charge designations are referenced in the bar at the bottom. Each graphic is the same model with different orientations rotated on the Y-axis. Graphics were created in PyMol.