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. Author manuscript; available in PMC: 2023 Jan 20.
Published in final edited form as: Biochemistry. 2021 Nov 11;62(2):410–418. doi: 10.1021/acs.biochem.1c00451

Figure 2.

Figure 2.

Comparison of Br512 and Bst-LF in LAMP-OSD assays of DNA templates. LAMP-OSD assays for the human GAPDH gene were carried out with 20 pmol of in-house purified Bst-LF (panel A) or 20 pmol of Br512 (panel B) in the indicated reaction buffer. Amplification curves were observed in real-time at 65 °C by measuring OSD fluorescence in reactions seeded with 600,000 (black traces), 60,000 (red traces), 6000 (blue traces), 600 (pink traces), and 0 (gray traces) copies of GAPDH plasmid templates.