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. 2022 Jul 27;8(5):2092–2103. doi: 10.1002/vms3.891

TABLE 1.

Specifications of primers used in RT‐PCR reaction

Gene NCBI accession number

Primer forward (5′to 3′)

Primer reverse (5′ to 3′)

Annealing temperature (°C) Amplicon length
Map2k1 NM‐001082629

CTGCTGGATTACATCGTCAA

CTGCTTCAAGTCTGCTCTC

60 126
Cdk2 XM‐002711062

GCATTAGAGGCAGGTGAGAG

AAAGACAGGAGCAGGGATTC

60 89
GAPDH NM‐001022531

TGAACCACGAGAAGTATGACAA

CCTCCACAATGCCGAAGT

60 114