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. 2022 Sep 27;20(9):e3001781. doi: 10.1371/journal.pbio.3001781

Fig 5. Auxin-signaling-dependent vein patterning and regulated PD aperture.

Fig 5

(A–S) Confocal laser scanning microscopy. First leaves (for simplicity, only half-leaves are shown in L–O, S). Blue, autofluorescence; yellow (A–K) or green (L–O), GFP expression; magenta, YFP signals. Dashed white line delineates leaf outline. (L, M) Side view, adaxial side to the left. (P–S) DR5rev::nYFPHS (P,Q) or DR5rev::nYFPES (R,S) expression; look-up table (ramp in Q) visualizes expression levels. Top right: leaf age in DAG, genotype, and treatment (10 or 50 μM PBA). Bottom left (A–K, P–S) or center (L–O): reproducibility index (see S1 Table). Images in P and R were acquired by matching signal intensity to detector’s input range (approximately 1% saturated pixels). Images in P and Q were acquired at identical settings and show weaker and broader DR5rev::nYFPHS expression in cals3-2d. Images in R and S (left) were acquired at identical settings and show weaker DR5rev::nYFPES expression in gsl8-2. Image in S (right) was acquired by matching signal intensity to detector’s input range (approximately 1% saturated pixels) and shows broader DR5rev::nYFPES expression in gsl8-2. Bars: (A–K) 120 μm; (L, M) 20 μm; (N–S) 80 μm. cals3-2d, callose synthase - 2 dominant; DAG, days after germination; DR5rev, direct repeat 5 reverse; erGFP, endoplasmic-reticulum-localized GFP; gsl8-2, glucan-synthase-like - 2; nYFP, nuclear YFP; PBA, phenylboronic acid; PD, plasmodesma.