Fig 5. Human HCT116 cells internalize bacterial OMVs.

Confocal microscopy imaging of human HCT116 cells stained with cell tracker CMTPX (in red) and incubated with bacterial OMVs labelled with PKH67 (in green) for 2 h (A) or 18 h (B). Nuclei were stained with DAPI (in blue). The XZ and YZ projection of volocity shows that bacterial OMVs were internalized by HCT116 cells and localized mainly to the cytoplasm (see S5 Fig).