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. 2022 Sep 15;18(9):e1010827. doi: 10.1371/journal.ppat.1010827

Fig 6. Bacterial OMVs deliver Ile-tRF-5X to human HCT116 cells.

Fig 6

Human HCT116 cells were incubated with (10 ng) E. coli MG1655-derived OMVs or transfected with an equivalent amount of synthetic Ile-tRF-5X. The level of Ile-tRF-5X was measured at 24 h and 48 h by LNA RT-qPCR. UniSp6 was used as spike-in control and reference gene for normalization. The results were reported in fold change compared to the corresponding mock condition. Statistical analysis. Data were calculated from three biological replicate measurements (n = 3; mean ± SD). One-way analysis of variance (ANOVA) and Holm-Šídák’s multiple comparisons test (post-hoc test) were used for statistical analysis. Statistically significant differences is indicated as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.