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. 2022 Sep 15;18(9):e1010827. doi: 10.1371/journal.ppat.1010827

Fig 9. Bacterial OMVs and Ile-tRF-5X modulate human MAP3K4 expression through the miRNA pathway.

Fig 9

(A) Dicer deletion (Dicer -/-) in human HCT116 cells was confirmed at the mRNA level by qPCR and at the protein level by Western blot using anti-Dicer and anti-Tubulin (control) antibodies. (B) Relative MAP3K4 mRNA expression was quantified at 48h by RT-qPCR in either wild-type (WT) or Dicer -/- HCT116 cells after incubation with bacterial OMVs (10 ng) or transfection with Ile-tRF-5X (100 nM). Data were normalized with a reference gene (ACTB), reported as fold change vs mock control, and expressed with the relative quantitation method (ΔΔCt). Statistical analysis. Data were calculated from three biological replicate measurements (n = 3; mean ± SD). Unpaired Student’s t test (A) and two-way analysis of variance (ANOVA) and Holm-Šídák’s multiple comparisons test (B) were used for statistical analysis. Statistically significant differences (fold change vs. mock) are indicated as follows: * p < 0.05; *** p < 0.001; **** p < 0.0001.