Figure 3. Slowing was specific to replay vs non-replay, and local replay vs remote.

(a) Examples of a replay (left) and two non-replays (right; absolute weighted correlation > 0.6 and maximum jump distance < 40% of the track).

(b) The duration of replay across passes for all replays (black) and all non-replays (gray). Non-replays did not increase in duration (N = 6893, R = 0.014, P = 0.24). Two-way ANOVA between group and pass: main effect of group: F_(1,8501) = 18.6, P = 1.7e-5, main effect of pass: F_(1,8501) = 30.1, P = 3.3e-8, interaction: F_(1,8501) = 10.9, P = 9.9e-4.

(c) Schematic of behavior for local vs non-local experiment: rats ran Session 1 on novel Track A, rested on a familiar platform, and then ran Session 2 on a different novel Track B.

(d) The duration of local replays increased with passes of the track (One-tail test: N = 1069, Duration: R = 0.13, P = 8.6e-6).

(e) The duration of remote replays of Track A did not increase with passes of Track B (One-tail test: N = 157, R = −0.14, P = 0.96). Two-way ANOVA local replay and remote replay: main effect of group P > 0.9, main effect of pass F_(1,1225) = 5.3, P = 0.021, interaction: F_(1,1225) = 10.3, P = 1.3e-3. Additionally, the duration of Track A replays at the end of session 1 was not different than at the beginning of session 2 (Session 1 local replays > 10 passes N = 856, Session 2 remote replays passes 1–4 N = 33; Wilcoxon rank-sum test, duration: P = 0.3). All data show mean ± S.E.M. Correlations are Pearson’s correlation values. *** P < 0.001, ** P < 0.02, * P < 0.05