FIGURE 8.

Flat mount of lens epithelial cell monolayer. (A) Lens capsule flat mount was stained for E-cadherin (red) and nuclei (blue). Tiled Z-stack scans from the anterior (top) to the meridional rows at the equator (bottom) were stitched, and this image is a maximum intensity projection of the stitched Z-stacks compressed into a 2D image. Scale bar, 50 μm. (B) Magnified images of epithelial cells from different regions of the lens. As expected, E-cadherin outlines the membrane of the epithelial cells and has reduced signals after epithelial cells differentiate into fiber cells at the lens fulcrum. It should be noted that the spacing of the nuclei changes slightly between anterior vs. equatorial epithelial cells. Scale bar, 10 μm.