FIG. 2.
Expression of fosB depends on ςW. Primer extension analysis of the fosB transcription start site is shown. RNA was isolated from logarithmically growing cells in Luria-Bertani medium of strains CU1065 (wild type), HB0020 (sigW mutant), and HB0010 (rsiW mutant). Equal amounts (100 μg) of total RNA were annealed with radiolabeled oligonucleotide no. 370 prior to analysis by reverse transcriptase primer extension. The transcription start site is the G residue shown in bold. G,A,T, and C are the sequencing ladder obtained with the same primer.