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. 2022 Feb 16;35(10):1431–1438. doi: 10.1038/s41379-022-01016-8

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© The Author(s) 2022

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Fig. 5 — A 3D reconstruction of nuclei in a CD8-stained (red) thick section of an NLPHL pattern A case. Nuclei were counterstained with DAPI (blue). Selected nuclei of T cells are highlighted by arrows. 63x magnification. B 3D reconstruction of nuclei in a CD8-stained (red) thick section of a THRLBCL case. Nuclei were counterstained with DAPI (blue). Selected nuclei of T cells are highlighted by arrows. 63x magnification. C Nuclear volumes of CD3⁺, PD1⁺ and CD8⁺ T cells in NLPHL pattern A, THRLBCL and PTGC (*p < 0.05, **p < 0.01, Kruskal–Wallis-Test with Dunn’s post-test) quantified in 3D immunostained thick sections.