Fig. 2.

HC-1119 treatment inhibited the increase in AR protein elevated by DHT treatment in AR-positive TNBC cells Hs578T (a) and SUM159PT (b) cells were cultured in a medium with 10% CSS for 48 h, and then treated with vehicle, 5 nM of DHT, 0.5 μm (for Hs578T cells) or 10 μm (for SUM159PT cells) of HC-1119, or both DHT and HC-1119 as indicated for 24 h. Whole cell lysates were prepared from the treated cells and analyzed by Western blot using AR antibody. GAPDH served as a loading control. Experiments were repeated at least three times and the representative bands of Western blot assays are presented. *, p < 0.05 by One-Way ANOVA