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. 2022 Aug 12;49(10):9231–9240. doi: 10.1007/s11033-022-07749-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — HC-1119 treatment suppresses DHT-induced nuclear localization of AR in AR-positive TNBC cells Hs578T cells were cultured in a medium with 10% CSS for 48 h, and then seeded onto glass coverslips in a 24-well plate, and treated for one hour with vehicle (Ctrl), DHT (5nM), HC-1119 (0.5 μm), or both DHT and HC-1119 as indicated. Treated cells were processed for immunecytofluorescence staining using AR antibody. Images were recorded with a confocal microscope