Table 2.
Percentage of polychromatic erythrocytes (PCEs) and PCEs with micronuclei (MPCEs); chromosomal aberrations induced in mouse bone marrow cells after treatment with carmustine and Codiaeum variegatum
| Treatment and doses | No. and percentage of PCEs/(PCEs + NCPs) | No. and percentage of MPCEs | Total abnormal metaphases | No and (%) of metaphases with different types of chromosome aberrations | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| NO | Mean ± S.E | NO | Mean ± S.E | No | Mean (%) ± SE | Gap | Fragment and/or break | Multiple aberrations | R.T | |
| I. Control (30% ethanol) | 419 | 4.19 ± 0.37a | 11 | 2.63 ± 0.63a | 23 | 4.60 ± 0.4 a |
7 (1.40) |
16 (3.20) |
– | – |
| II. Carmustine (30 mg / kg) | 1448 | 14.48 ± 0.49d | 143 | 9.88 ± 0.58c | 163 | 32.60 ±0.0.75 e |
12 (2.40) |
42 (8.40) |
102 (20.40) |
7 (1.40) |
| III. Codiaeum variegatum (500 mg/kg) | 438 | 4.38 ± 0.54a | 13 | 2.97 ± 0.27a | 16 | 3.20 ± 0.58a |
7 (1.40) |
9 (1.80) |
– | – |
| IV-VI- Carmustine + Codiaeum variegatum | ||||||||||
| + 100 mg/kg | 1132 | 11.32 ± 0.53c | 88 | 7.77 ± 0.61 c | 130 | 26.0 ± 0.89d |
14 (2.80) |
37 (7.40) |
72 (14.40) |
7 (1.40) |
| + 300 mg/kg | 1005 | 10.05 ± 0.49b | 67 | 6.67 ± 0.49 b | 107 | 21.40 ± 0.98 c |
13 (2.60) |
41 (8.20) |
49 (9.80) |
4 (0.80) |
| + 500 mg/kg | 887 | 8.87 ± 0.32b | 51 | 5.75 ± 0.26 b | 86 | 17.20 ± 0.86b |
11 (2.20) |
35 (7.0) |
36 (7.20) |
4 (0.80) |
A total of 500 cells were analyzed (5 mice per group; 100 cells/mouse) for chromosomal aberrations. R.T.: Robertsonian translocation. No. of examined nucleated cells = 2000/mouse (5 mice/group) for micronuclei test.One way ANOVA–Tukey’s multiple comparisons test was used. The values having different superscript letters in each column are significantly different from one another at p < 0.05