Fig. 3.

DUXAP10 promotes OS cell migration and invasion, inhibits cell apoptosis, and affects cell cycle progression in vitro. A Transwell chamber assay was used to detect the effect of DUXAP10 on OS cell migration ability. B Transwell chamber with Matrigel matrix assay was used to detect the effect of DUXAP10 on OS cell invasion ability. C Flow cytometry assay was used to detect the effect of DUXAP10 on OS cell cycle; Western blot assay was used to detect the change of cell cycle-regulated proteins expression in DUXAP10 knockdown U2OS cells. D Flow cytometry assay was used to detect the effect of DUXAP10 on OS cells apoptosis; Western blot assay was used to detect the change of cell apoptosis-regulated proteins expression in DUXAP10 knockdown U2OS cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001