Fig. 5.

SOX18 is identified as a critical downstream target of DUXAP10 in OS cells. A Transcriptome high-throughput sequencing revealed differentially expressed mRNAs in DUXAP10 knockdown U2OS cells. B qRT-PCR assay was used to detect the changes of mRNA expression in U2OS and SAOS2 cells. C Western blot assay was used to detect the expression of SOX18 at protein level in U2OS and SAOS2 cells. D Expression of SOX18 in OS and normal bone tissues we collected. E Correlation analysis was used to detect the relation between DUXAP10 and SOX18 in OS tissues. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001