Fig. 6.

DUXAP10 specifically binds to HuR in OS cells. A Nucleocytoplasmic separation assay was used to detect the distribution of DUXAP10 in OS cells. B We predicted the binding ability of HuR to DUXAP10 by the online website (http://pridb.gdcb.iastate.edu/RPISeq). C qRT-PCR and Western blot assays were used to detect the expression of SOX18 in HuR knockdown U2OS and SAOS2 cells. D RIP assay was used to detect the degree of binding of HuR to DUXAP10 and SOX18. E qRT-PCR assay was used to detect the percentage of SOX18 mRNA that remained treated with actinomycin D in U2OS cells. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01, ***P < 0.001