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. 2022 Sep 27;8:112. doi: 10.1038/s41523-022-00480-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Frequency of enumerated LEVs between late-stage, early-stage, and the normal group based on channel classification. b Comparison of the distribution of LEVs between groups, Kruskal-Wallis H test (one-way ANOVA) performed. All p values below *0.05 considered statistically significant. c Size comparison of LEVs and rare cell events. All sizes represent diameters in micron. Sizes calculated by feature conversion from 100x images. d Heatmap displaying signal intensity of biomarkers on LEVs and DAPI + PanCK+ cells. e Scaled frequency plots of rare cells and LEVs in patients, designated colors represent classification groups marked in the figure on panel c.