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. 2022 Sep 27;8:112. doi: 10.1038/s41523-022-00480-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a–c Blood specimens are collected, processed, and plated onto slides, and undergo immunofluorescent staining. d Slides are scanned, acquired images are segmented, cellular features are extracted using R and EBImage software, dimensionality reduction analysis is applied to the cells. e Data processing pipeline allows for rare cell detection, filtering, and classification, and DAPI- event separation for curation of final report. Created with BioRender.com.