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. 2022 May 3;64(11):1227–1233. doi: 10.1007/s12033-022-00500-5

Fig. 2.

Fig. 2

Schematic representation of one-step genotyping of the tetra-primer PCR system. The tetra primers are composed of a pair of common primers, a wt-specific primer and a loxP-specific primer. The green block is the common primer amplified sequence; the blue block is the wt-specific primer amplified sequence; the orange block is the loxP-specific primer amplified sequence; the red block is the loxP site