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. 2022 May 3;64(11):1227–1233. doi: 10.1007/s12033-022-00500-5

Table 1.

Sequences of primers used for polymerase chain reaction

Gene Primer sequence (5'‑3') Tm GC%
Mbd2_Com_F CCTGCTCGTTGACAGGGTTATC 61.2 54.5
Mbd2_Com_R GGTCAACAGCATTTCCCAGGTA 61.5 50.0
Mbd2_wt_R GCGTTTGGAATGCACATAGTCTG 62.3 47.8
Mbd2_loxP_F GTTATAAGCCTATACCAGACATAACTTCGT 59.6 35.7
Pdia3_Com_F GCTGGAGTCTATTCCCTGTGTGT 59.9 52.2
Pdia3_Com_R GCTTACTGCTAAGCCTAAGGACTCA 61.2 48.0
Pdia3_loxP_F GATATAGCATCCTACAGTCAGTGCAAAT 62.1 39.3
Pdia3_wt_R TCACCCTTTTCCACCTCTATTGC 62.4 47.8
Wtap_Com_F CACTTGGACGGCTCAAGGATG 62.7 57.1
Wtap_Com_R AGGGATGGCATAATGGTAAGTAGG 61.0 45.8
Wtap_wt_R TGGAACTCTTCAGAGAACCAGTTTAGT 61.7 40.7
Wtap_loxP_F TCTGACTGATTAGGTATTGCGAACTATAA 62.2 34.5