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. 2022 Sep 27;5:1018. doi: 10.1038/s42003-022-04004-5

Fig. 7. AMPK activation reduced the proliferation of wound epithelium.

Fig. 7

a Formation of wound epithelium (WE) in control (DMSO), mTOR inhibition (RAD001), and AMPK activation groups (A769662). b Number of geckos with regeneration in control, RAD001, and A769662 groups. N = 15. c H&E staining of tail tissues in control, RAD001, and A769662 groups. Solid arrow indicates the injury site, dashed box indicates the magnified area, dotted line indicates border between wound epithelium and scab, and solid line indicates junction between rostral end of wound epithelium and blastema. Scale bar = 500 µm or 100 µm. N = 3. d Protein level of AMPKα, p-AMPKα (Thr172), mTOR, and p-mTOR (Ser2448) in tail tissues. e–g Relative level of proteins in Fig. 7d. h Immunofluorescence analysis of BrdU staining in control, RAD001 and A769662 group (upper panel). Immunohistochemical analysis of WE6 staining in the same location of the samples. Solid line indicates the caudal border of wound epithelium, dotted line indicates border between wound epithelium and blastema. Scale bar = 50 µm. i Number of BrdU positive cells in control, mTOR inhibition, and AMPK activation groups. Values represent mean ± SD, N = 5, nsp > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001.