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. 2022 Sep 27;12:16126. doi: 10.1038/s41598-022-20507-x

Figure 2.

Figure 2

Temperature sensitivity of cna1 or cnb1 gene-deficiency in T. asahii. (a) The T. asahii parent strain (Parent), the cna1 gene-deficient mutant (∆cna1), the revertant of ∆cna1 (CNA1), the cnb1 gene-deficient mutant (∆cnb1), and the revertant of ∆cnb1 (CNB1) were grown on SDA and incubated at 27 °C for 2 days. T. asahii cells was suspended in physiologic saline solution and filtered through a 40-μm cell strainer. Series of tenfold dilution of the fungal suspension were prepared using saline. Five microliters of each cell suspension was spotted on the SDA. Agar plates were incubated at 27 °C, 37 °C, or 40 °C for 24 h. (b, c) The T. asahii parent strain (Parent), the cna1 gene-deficient mutant (∆cna1), the revertant of ∆cna1 (CNA1), the cnb1 gene-deficient mutant (∆cnb1), and the revertant of ∆cnb1 (CNB1) were inoculated on Sabouraud medium and incubated at 27 °C, 37 °C, or 40 °C. Absorbance of the culture at 630 nm was monitored. Data are shown as means ± standard error of the mean (SEM).