Figure 7.

Effect of cna1 or the cnb1 gene-deficiency on T. asahii morphology in human serum. The T. asahii parent strain (Parent), the cna1 gene-deficient mutant (∆cna1), the revertant of ∆cna1 (CNA1), the cnb1 gene-deficient mutant (∆cnb1), and the revertant of ∆cnb1 (CNB1) were grown on SDA and incubated at 27 °C for 2 days. T. asahii cells was suspended in physiologic saline solution and filtered through a 40-μm cell strainer. Absorbance at 630 nm of the T. asahii cell suspension was adjusted to 1–1.5. The cell suspension (10 µl) was added to human serum (100 µl) and the solutions were incubated at 37 °C for 48 h. The incubated solution was placed on glass slides and covered by a glass coverslip. (a) Samples were examined with bright light under a microscope. The scale bar indicated 50 µm. (b) The pictures were randomly obtained. The numbers of three cell types: yeast + blastoconidia, arthroconidia, and hyphae, were counted.