Figure 7.

A spontaneous phospholipid translocation event observed in the simulation of mTMEM16F F518K/Y563K mutant. The z-distance of the lipid headgroup center of mass to the membrane center is shown as the black line as a function of simulation time. The z-position was analyzed every 1 ns (gray trace), and 20 ns running average is plotted. The phospholipid orientations at various time points are illustrated as red arrows, with the headgroup being the arrow head and the tail as the arrow tail. The lipid headgroup contacting residues along the trajectory are shown by different colors and shapes drawn at corresponding z-positions. To see this figure in color, go online.