FIGURE 4.

Immunohistochemistry staining and flow cytometry analysis of blood and brain CD8⁺ T cells. (A, C, and E) Representative images of hippocampal CD8⁺ T cells of APP-PS1 mice. Spleen tissue was used as a positive control for Ab stainings. DAPI (blue) was used as a nucleus stain and thioflavin S (white) was used to visualize amyloid plaques. Scale bars, 30 µm, inserts 10 µm. (B, D, and F) Flow cytometry analysis of CCR7, CD62L, and CD103 coexpression on CD8⁺ T cells in blood and brain of WT and APP-PS1 mice (n = 7–8). Statistical differences between the groups were identified via an ordinary one-way ANOVA, followed by Šídák’s multiple comparisons test. A p value <0.05 was considered statistically significant.