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. 2022 Sep 14;12:988355. doi: 10.3389/fcimb.2022.988355

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Copyright © 2022 Ding, Yang, Jiang, Zhou, Li and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of ASFV p30 and p54 as fusion proteins in HEK-293T cells. (A) A schematic representation of the plasmids expressing the fusion proteins, GLuc-p30 and GLuc-p54. HEK-293T cells were transfected with pCAGGS-GLuc-p30, pCAGGS-GLuc-p54, or pCAGGS vector. At 24, 48, and 72 hpt, culture supernatants were harvested, while cells were lysed with IP lysis buffer or fixed with 4% paraformaldehyde. The expression of GLuc-p30 and GLuc-p54 at 48 hpt was evaluated by IFA using an antibody against the FLAG tag (B) and western blot analysis using antibodies against the FLAG tag and GAPDH (C). Luciferase assay was performed to measure GLuc-p30 (D) and GLuc-p54 (E) in cell lysates and supernatants at 24, 48, and 72 hpt. (F) The ratio of secreted GLuc-p30 and GLuc-p54. The data (E, F) represent the means ± SD (standard deviation) of triplicate.