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. 2022 Sep 28;30(11):1518–1526.e4. doi: 10.1016/j.chom.2022.09.015

Figure 3.

Figure 3

BA.2.75 exhibits enhanced cell-cell fusion and S processing

(A) Fluorescence images displaying syncytia formation are presented for HEK293T-ACE2 cells 48 h after co-transfection with a GFP expression construct and SARS-CoV-2 variant S proteins. Scale bars represent 150 μm.

(B) Quantification of syncytia formation in (A) displays the mean syncytia size; bars represent mean ± standard error, with significance relative to D614G determined by one-way ANOVA with Bonferroni multiplicity correction. p values are displayed as ∗∗∗∗p < 0.0001.

(C) Histogram displays of the surface staining of HEK293T cells expressing S proteins, which were detected by an anti-S1 antibody (T62).

(D) Quantification of relative surface expression as shown in (C); bars represent mean ± standard error.

(E) Pseudotyped lentivirus producer cell lysate was assessed for processing of S by probing with anti-S1 (T62), anti-S2, anti-HIV-1 Gag (anti-p24), and anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase). Band intensities were quantified in ImageJ and the ratio of S1/S or S2/S is displayed relative to the S1/S or S2/S ratio of BA.2.