REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-SARS-CoV-2 S1	Sino Biological	Cat# 40150-T62, RRID:AB_2920715
anti-SARS-CoV-2 S2	Sino Biological	Cat# 40590-T62, RRID: AB_2857932
anti-p24	NIH	Cat# ARP-1513, RRID: AB_2832923
anti-GAPDH	Santa Cruz Biotechnology	Cat# sc-47724, RRID: AB_627678
anti-Rabbit IgG (whole molecule)–FITC antibody	Sigma-Aldrich	Cat# F9887, RRID: AB_259816
anti-Mouse IgG (whole molecule)–Peroxidase	Sigma-Aldrich	Cat# A5278, RRID: AB_258232
anti-Rabbit IgG (whole molecule)–Peroxidase	Sigma-Aldrich	Cat# A9169, RRID: AB_258434
Bacterial and virus strains
E.coli DH5α	Invitrogen	Strain #: DH5α
E.coli Stable 2	Invitrogen	Strain #: Stable 2
Biological samples
2-dose HCWs Sera	(Evans et al., 2022; Qu et al., 2022a)	N/A
3-dose HCWs Sera	(Evans et al., 2022; Qu et al., 2022a)	N/A
Omicron-wave Infected Patient Sera	(Evans et al., 2022; Qu et al., 2022a)	N/A
Chemicals, peptides, and recombinant proteins
Polyethylenimine (PEI)	Thermo Fisher Scientific	Cat# BMS1003
Dulbecco’s Modified Eagles Medium (DMEM)	Sigma-Aldrich	Cat#: 11965-092
Fetal Bovine Serum (FBS)	Thermo Fisher Scientific	Cat#: F1051
0.05% Trypsin + 0.53 mM EDTA	Corning	Cat# 25-052-CI
Penicillin-Streptomycin	HyClone	Cat#: SV30010
Protease Inhibitor Cocktail	Sigma-Aldrich	Cat# P8340
Immobilon Crescendo Western HRP substrate	Millipore	Cat# WBLUR0500
QIAprep Spin Miniprep Kit	QIAGEN	Cat# 27106
Coelenterazine	GoldBio	Cat#: CZ2.5, CAS: 55779-48-1
Deposited data
NT50 Values and De-identified patient data	SeroNet Coordinating Center, NCI, NIH	N/A
Experimental models: Cell lines
HEK293T	ATCC	Cat#: CRL-11268, RRID: CVCL_1926
HEK293T-ACE2	BEI Resources	Cat#: NR-52511, RRID: CVCL_A7UK
Recombinant DNA
pNL4-3-inGluc	David Derse, NCI, NIH (Mazurov et al., 2010)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_D614G	GenScript Biotech (Evans et al., 2022)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.1	GenScript Biotech (Evans et al., 2022)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.12.1	GenScript Biotech (Qu et al., 2022)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.4/5	GenScript Biotech (Qu et al., 2022)	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75	GenScript Biotech This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_K147E	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_W152R	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_F157L	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_I210V	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_G257S	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_D339H	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_G446S	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_N460K	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2_R493Q	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_E147K	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_R152W	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_L157F	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_V210I	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_S257G	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_H339D	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_S446G	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_K460N	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2.75_Q493R	This paper	N/A
pcDNA3.1-SARS-CoV-2-Flag-S-Flag_BA.2	GenScript Biotech (Evans et al., 2022)	N/A
Software and algorithms
GraphPad Prism	Version 9.0.0	GraphPad
ImageJ	(Schneider et al., 2012)	https://imagej.nih.gov/ij/
FlowJo	FlowJo, LLC	https://www.flowjo.com/
SWISS-MODEL	(Guex et al., 2009)	https://swissmodel.expasy.org/
programs Coot	(Emsley et al., 2010)	https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/
PyMOL	Warren DeLano and Sarin Bromberg	https://pymol.org/
Leica Application Suite X	Leica Microsystems	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/
Other
Cytation 5 Imaging Reader	BioTek	N/A
L-70 Optima Ultracentrafuge	Beckman	N/A
Amersham Imager 600	GE Healthcare Life Sciences	N/A