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. 2022 Sep 14;13:961788. doi: 10.3389/fphar.2022.961788

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Copyright © 2022 Wu, Qi, Wang, Wang, Sun, Dong, Yu, Gao, Zhang and Tian.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro cellular uptake and drug detention studies in a co-cultured system. (A) Cell uptake model of BEL-7402 and LX-2 cells. (B) Drug retention assay model. CLSM images of BEL-7402 cells (C) and LX-2 cells (D) treated with C6/Lip, C6/GA-Lip, and C6/GA-HA-Lip at 2 h, where the nuclei were labeled with DAPI (blue) and Lip were labeled with C6 (green); scale bars represent 50 μm. (E) Confocal microscopy and (F) quantitative analysis of drug retention were used to detect drug retention at 4, 12, and 24 h; scale bar represents 50 µm.