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. 2022 Sep 14;13:975233. doi: 10.3389/fimmu.2022.975233

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Copyright © 2022 Falcon, Smith, Al-Obaidi, Abu Zaanona, Purvis, Minagawa, Athar, Salzman, Bhatia, Goldman and Di Stasi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Mitochondrial dysfunction assessed with the MytoSox Red flow cytometry method on ΔiC8-RQR8, Rapa.ΔiC9a-ΔCD19, or F36VΔiC8/RapaΔiC9 co-expressing cells treated with the BB homodimerizer, sirolimus (siro) or both. (B): Day 14 analysis of regrowth (N: 3) of ΔiC8-RQR8/Rapa.ΔiC9 suicide gene-modified Jurkat cells treated with the BB homodimerizer, sirolimus (siro) or both. (C) Titration of ΔiC8-RQR8/Rapa.ΔiC9 suicide gene-modified Jurkat cells with decreasing concentration of BB homodimerizer or sirolimus.