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. 2022 Sep 14;13:970139. doi: 10.3389/fmicb.2022.970139

FIGURE 6.

FIGURE 6

Tomato yellow leaf curl virus (TYLCV) levels were affected by silencing essential genes in the salicylic acid (SA) and jasmonate acid (JA) signaling pathways. (A) The first picture (from left to right) shows the negative control plant carrying pTRV1 + empty pTRV2. The second picture shows the positive control plant carrying pTRV1 + pTRV2-SlPDS. The bleached areas in leaves were visible 2 weeks after Agro-inoculation. The third picture shows plants Agro-infiltrated with pTRV1 + pTRV2-SlNPR1 while the forth picture depicts plants Agro-infiltrated with pTRV1 + pTRV2-SlCOI1. (B) The relatively expression levels of SlNPR1 in pTRV1 + pTRV2-SlNPR1 Agro-inoculated plants. (C) The relatively expression levels of SlCOI1 in pTRV1 + pTRV2-SlCOI1 Agro-inoculated plants. The SlNPR1 or SlCOI1 expression profile in the pTRV1 + empty pTRV2 inoculation plants (CK) was set to 1 and served as the baseline. Shown is representative data from three independent biological replicates. (D,E) The relative levels of TYLCV in pTRV1pTRV2-SlNPR1 plants and pTRV1 + pTRV2-SlCOI1 plants 7 days post inoculation of TYLCV. CK represents the baseline TYLCV levels in plants with pTRV1 + empty pTRV2 agroinfiltration. The mean and SEM are derived from pooled data corresponding to three independent biological replicates.