Figure 4.

Corynebacterium accolens cell-free culture supernatants reduce S. aureus cell-free culture supernatants-induced detrimental effects on HNEC-ALI cultures tight junctions. Immunofluorescence staining of tight junction proteins of HNEC-ALI cultures treated with cell-free culture supernatants from SA and SC co-cultured with C. accolens in different ratios. HNEC-ALI cultured cells were stained with antibodies against Z0-1(green), claudin-1 (red) and DAPI to resolve nuclei (blue). TSB treatment was used as the negative control. Triton-100 was used as the positive control. Images were examined with confocal laser-scanning microscope (Scale bar=10 μm). C1, C. accolens clinical isolate 1; SA, S. aureus ATCC51650; SC, S. aureus clinical strain.