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. 2022 Sep 14;13:953225. doi: 10.3389/fpls.2022.953225

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Copyright © 2022 Kim, Jang, Seomun, Yoon and Jang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Abnormal root hair patterning in CO2::ΔARF5 plants. (A) Schematic diagram of root hair patterning in Arabidopsis roots. Blue and pink indicate hair cells (H) and non-hair cells (N), respectively. (B) Root hair patterning in wild-type and CO2::ΔARF5 transgenic plants grown in 1/2 MS solid medium for 7 days. #1 and 2 indicate two independent lines of CO2::ΔARF5 plants. Black arrows point to the formation of double H files in CO2::ΔARF5 plants. Scale bars = 20 μm. (C) Quantification of abnormal root hair patterning in these plants (n > 30). Error bars represent SD, and asterisks indicate a significant difference from the wild-type control (p < 0.01, two-tailed t-test).