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. 2022 Jul 21;34(10):4088–4104. doi: 10.1093/plcell/koac209

Figure 1.

Figure 1

PAMP treatment induces phosphorylation of CBP60g. A, Flg22 induces phosphorylation of CBP60g in protoplasts. Col-0 protoplasts were transfected with Pro35S:CBP60g-FLAG and treated with or without 2 µM flg22. Total protein extracted from protoplasts was treated with or without 2 unit/µL PPase as indicated. B, Flg22 induces phosphorylation of CBP60g in transgenic plants. Pro35S:CBP60g-FLAG transgenic plants were treated with H2O or 2 µM flg22 for 5 min and total protein extracted from leaves was treated with or without 2 unit/µL PPase as indicated. C and D, Chitin or nlp20 induces phosphorylation of CBP60g in transgenic plants. Pro35S:CBP60g-FLAG transgenic plants were treated with H2O, 250 µg/mL chitin, or 10 µM nlp20 as indicated for 5 min. Total protein was extracted from treated leaves. Protein samples were separated by Phos-tag SDS-PAGE and subjected to anti-FLAG immunoblot. Coomassie brilliant blue staining of the filter indicates loading of the protein. The experiments were repeated 3 times with similar results.